DAPT, gamma-Secretase inhibitor from Abcam

Product Specs
ItemDAPT, gamma-Secretase inhibitor
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab120633
Quantity10 MG, 50 MG
Purity0.99
Typeinhibitor

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Abcam

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Cambridge Biomedical Campus

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United Kingdom

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+44 (0) 1223 696000 (option 2)
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Website: http://www.abcam.com

(1) Stepwise emergence of the neuronal gene expression program in early animal evolutionCellOctober 12, 2023Sebastián R. Najle, Xavier Grau-Bové, Anamaria Elek, Cristina Navarrete, Damiano Cianferoni, Cristina Chiva, Didac Cañas-Armenteros, Arrate Mallabiabarrena, Kai Kamm, Eduard Sabidó, et al.Tools,Cat#1128-25 Methyltetrazine-DBCO,Click Chemistry Tools,Cat#1022-10 DRAQ5,Thermo Scientific,Cat#62251 DAPT,Abcam,Cat#ab120633 LY411575,SelleckChem,Cat#S2714 Yhhu3792,Tocris,Cat#6599 Membrite-Fix 488/515,Biotum,Cat#30093-T Proteinase-K,New England BioLabs

(2) Calcium dysregulation combined with mitochondrial failure and electrophysiological maturity converge in Parkinson’s iPSC-dopamine neuronsiScienceJuly 21, 2023Dayne A. Beccano-Kelly, Marta Cherubini, Yassine Mousba, Kaitlyn M.L. Cramb, Stefania Giussani, Maria Claudia Caiazza, Pavandeep Rai, Siv Vingill, Nora Bengoa-Vergniory, Bryan Ng, et al.dihydrochloride),Bio-Techne,Cat#1254 GDNF,Peprotech,Cat#450-10 TGFb3,Peprotech,Cat#100-36E DAPT,Abcam,Cat#ab120633 Ascorbic acid,Sigma,Cat#A4544 LDN-193189,Sigma,Cat#SML0559 SB-431542,Bio-Techne,Cat#1614 SHH C24II,Bio-Techne,Cat#1845-SH-500 Purmorphamine,Bio-Techne,Cat#4551/10 FGF8a,Stratech,Cat#16124-HNAE-SIB CHIR-99021,Bio-Techne,Cat#4423 BDNF,Peprotech,Cat#450-02 (db)-cAMP,Sigma,Cat#D0627 hESC-qualified

(3) Notch4 participates in mesenchymal stem cell-induced differentiation in 3D-printed matrix and is implicated in eccrine sweat gland morphogenesisBurns & TraumaJune 29, 2023Yuzhen Wang, Fanliang Zhang, Bin Yao, Linhao Hou, Zhao Li, Wei Song, Yi Kong, Yaxin Tan, Xiaobing Fu, Sha Huangdecreased Notch intracellular domain [25]. To inhibit Notch signaling in cultured cells, DAPT powder (ab120633, Abcam) was dissolved in dimethyl sulfoxide (DMSO) to 100 mM as stock solution and then were added into

(4) Generation of multilineage liver organoids with luminal vasculature and bile ducts from human pluripotent stem cells via modulation of Notch signalingStem Cell Research & TherapyFebruary 3, 2023Hyo Jin Kim, Gyeongmin Kim, Kyun Yoo Chi, Hyemin Kim, Yu Jin Jang, Seongyea Jo, Jihun Lee, Youngseok Lee, Dong-Hun Woo, Choongseong Han, et al.Sigma), 1.25 mM N-acetyl-L-cysteine (A9165, Sigma), 0.5 μM A83-01 (2939, Tocris Bioscience), 10 μM DAPT (ab120633, Abcam), 3 μM dexamethasone (D4902, Sigma), 0.566 mg/ml growth factor-reduced Matrigel (M354230, Corning),

(5) M6A associated TSUC7 inhibition contributed to Erlotinib resistance in lung adenocarcinoma through a notch signaling activation dependent wayJournal of Experimental & Clinical Cancer ResearchDecember 1, 2021Kai Li et al.atmosphere with 5% CO2. Materials and agents The Notch inhibitors were chosen as FLI-06 (inhibitor-1, ab120633, Abcam), and γ-Secretase inhibitor (inhibitor-2, ab146170, Abcam). Erlotinib was purchased from Sigma-Aldrich

(6) Effect of the Notch4/Dll4 signaling pathway in early gestational intrauterine infection on lung developmentExperimental and Therapeutic MedicineJuly 8, 2021Canyang Zhan, Yi Sun, Jiarong Pan, Lihua Chen, Tianming Yuan(Invitrogen; Thermo Fisher Scientific, Inc.) for 24 h. PMVECs were pretreated with 10 µmol/l DAPT (cat. no. ab120633; Abcam) or equal volume of vehicle PBS for 24 h according to previous studies (14,15). Subsequently,

(7) Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assaysSTAR ProtocolsJune 1, 2021Lay Shuen Tan, Resilind Su Ern Chew, Natasha Hui Jin Ng, Adrian Kee Keong Teo# 4423 Collagenase type IV,Thermo Fisher Scientific (Invitrogen),Cat # 17104019 DAPT,Abcam,Cat # AB120633 Dimethyl sulfoxide (DMSO),Sigma-Aldrich,Cat # D2650 Dispase in DMEM/F12,STEMCELL Technologies,Cat

(8) Alpha-synuclein dynamics in induced pluripotent stem cell-derived dopaminergic neurons from a Parkinson's disease patient (PARK4) with SNCA triplicationFEBS open bioFebruary 1, 2021Fukusumi H, Togo K, Sumida M, Nakamori M, Obika S, Baba K, Shofuda T, Ito D, Okano H, Mochizuki H, et al.Kyoto, Japan), 1 ng·mL−1 recombinant human TGF‐β3 (TGFβ3; cat. 100‐36E; PeproTech), and 10 µm DAPT (cat. ab120633; Abcam, Cambridge, UK) supplemented with 3 µm CHIR. On day 13, 75 µL of terminal differentiation medium

(9) Emodin inhibiting neutrophil elastase-induced epithelial-mesenchymal transition through Notch1 signalling in alveolar epithelial cellsJournal of cellular and molecular medicineOctober 1, 2020Zhou L, Gao R, Hong H, Li X, Yang J, Shen W, Wang Z, Yang J.inhibitor, was used to treat RLE‐6TN cells at a density of 100 µg/ml. The γ‐Secretase Inhibitors DAPT (ab120633, Abcam) was used as notch pathway inhibitor to RLE‐6TN cells at a density of 50 nM. 2.2 Immunoblotting

(10) Inflammasome Sensor NLRP1 Confers Acquired Drug Resistance to Temozolomide in Human MelanomaCancersSeptember 4, 2020Zhai Z, Samson JM, Yamauchi T, Vaddi PK, Matsumoto Y, Dinarello CA, Ravindran Menon D, Fujita M.dimethyl sulfoxide (DMSO), and stored at −20 ◦ C in aliquots. The γ-secretase inhibitor, DAPT, was purchased from Abcam (ab120633; Cambridge, MA, USA), reconstituted at a stock concentration of 50 mM in DMSO, and stored at −20 ◦ C

(11) BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cellsCell death & diseaseMay 18, 2020Loo LSW, Soetedjo AAP, Lau HH, Ng NHJ, Ghosh S, Nguyen L, Krishnan VG, Choi H, Roca X, Hoon S, et al.Technologies,11530-037 Collagenase IV,Life Technologies,17104019 DAKO mounting medium,DAKO,S3023 DAPI,Sigma,D9542 DAPT,Abcam,Ab120633 Dispase in DMEM/F12,STEMCELL Technologies,07923 D(+)-Glucose,WAKO,049-31165 Donkey serum,Merck,S-30 DMEM

(12) Xenotransplanted Human Cortical Neurons Reveal Species-Specific Development and Functional Integration into Mouse Visual CircuitsNeuronDecember 4, 2019Linaro D, Vermaercke B, Iwata R, Ramaswamy A, Libé-Philippot B, Boubakar L, Davis BA, Wierda K, Davie K, Poovathingal S, et al.DDM/B27+Nb/B27 medium. At 14-16 days after thawing, cells were treated with 10 μM DAPT (Abcam, Cat#ab120633) for 24 hours. The following day, cells were treated with 5 μM Cytarabine (Merck, Cat#C3350000) for

(13) Single-Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell PhenotypesCell stem cellJanuary 3, 2019Lang C, Campbell KR, Ryan BJ, Carling P, Attar M, Vowles J, Perestenko OV, Bowden R, Baig F, Kasten M, et al.C24II,Bio-Techne,Cat#1845-SH-500 Purmorphamine,Bio-Techne,Cat#4551/10 FGF8a,Stratech,Cat#16124-HNAE-SIB CHIR-99021,Bio-Techne,Cat#4423 BDNF,Peprotech,Cat#450-02 GDNF,Peprotech,Cat#450-10 TGFb3,Peprotech,Cat#100-36E DAPT,Abcam,Cat#ab120633 Ascorbic acid,Sigma,Cat#A4544 (db)-cAMP,Sigma,Cat#D0627 hESC-qualified Matrigel,Corning,Cat#354277 DQ

(14) Furin inhibitor protects against neuronal cell death induced by activated NMDA receptorsScientific ReportsDecember 1, 2018Mariko Yamada, Hideki Hayashi, Moe Yuuki, Nahoko Matsushima, Bo Yuan, Norio Takagiand Calpeptin (Cat. No. 03-34-0051) were procured from Millipore (Billerica, MA, USA). DAPT (Cat. No. ab120633) was purchased from Abcam (Cambridge, UK). Other cell culture reagents and medium were from Invitrogen

(15) Cellular aspect ratio and cell division mechanics underlie the patterning of cell progeny in diverse mammalian epitheliaeLifeJune 13, 2018McKinley KL, Stuurman N, Royer LA, Schartner C, Castillo-Azofeifa D, Delling M, Klein OD, Vale RD.inhibitor,Cayman,13013,200 µM Y27632,ROCK inhibitor,Selleck,S1049,10 µM DAPT,Gamma-secretase (Notch) inhibitor,Abcam,ab120633,50 µM Small molecules used in this study.

(16) Functional studies on the role of Notch signaling in Hydractinia developmentDevelopmental biologyAugust 1, 2017Gahan JM, Schnitzler CE, DuBuc TQ, Doonan LB, Kanska J, Gornik SG, Barreira S, Thompson K, Schiffer P, Baxevanis AD, et al.Notch signaling N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester (DAPT; Abcam, ab120633) was diluted to a stock concentration of 10 mM or 0.1 mM in DMSO and then diluted 1:1000 in artificial

(17) Basic fibroblast growth factor reduces scar by inhibiting the differentiation of epidermal stem cells to myofibroblasts via the Notch1/Jagged1 pathwayStem cell research & therapyMay 16, 2017Wang P, Shu B, Xu Y, Zhu J, Liu J, Zhou Z, Chen L, Zhao J, Liu X, Qi S, et al.PHG0264; Gibco), bFGF + SU5402 (10 μmol/L, PK-CA577-1645-05; PromoCell, Germany), bFGF + DAPT (20 μmol/L, ab120633; Abcam, UK), siJagged1, bFGF + siJagged1, and control. The control group was treated with K-SFM medium

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DAPT, gamma-Secretase inhibitor from Abcam

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DAPT, gamma-Secretase inhibitor from Abcam

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